Regulatory

Part:BBa_K2832001:Design

Designed by: Michael Taylor and Arianna Sigalos   Group: iGEM18_FSU   (2018-09-09)


PosmC - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PosmC_Design_Diagram.png PosmC Design Diagram. The osmC promoter sequence binds RcsB or NhaR. RcsB pathway is important for cell growth functions. NhaR is activated via increased concentration of soluble ions, possible through the opening of a mechanosensitive channel. mRFP1 signal indicates successful activation of the pathway. PosmC_Plasmid_Map.png

FSU Promoter BBa_K2832001 inside plasmid pSB1C3

Source

E. coli K12

===References===